Ordering Guidance

This test is intended for patient collection of vaginal specimens in a clinic/healthcare setting.

For clinician collected cervical specimens, order HPV / Human Papillomavirus (HPV) DNA Detection with Genotyping, High-Risk Types by PCR, ThinPrep, Varies.

For clinical collected vaginal specimens, order VHPV, Human Papillomavirus (HPV) Vaginal Detection with Genotyping for High-Risk Types by PCR.

Necessary Information

Specimen source is required.

Specimen Required

Patient Preparation: For 24 hours prior to specimen self-collection, patients should avoid using feminine hygiene products.

Supplies: Evalyn Brush (T990)

Specimen Type: Vaginal

Collection Container/Tube: Evalyn Brush

Submission Container/Tube: ThinPrep/PreservCyt solution vial

Specimen Volume: 3 mL of solution in ThinPrep/PreservCyt vial

Specimen Stability Information:

Evalyn Brush: Ambient 72 hours

ThinPrep/PreservCyt Vial: Ambient (preferred) 42 days/Refrigerated 42 days

Collection Instructions:

1. Specimen must be collected by the patient in a healthcare setting.

2. Provide patient with a labeled Evalyn brush and the accompanying self-collection instruction pamphlet.

3. Following patient self-collection, ensure that the Evalyn brush is tightly capped and appropriately labeled.

4. Place labeled Evalyn brush in a biosafety bag and send to the laboratory.

5. Perform the following steps prior to shipment to Mayo Clinic Laboratories:

 a. Label PreservCyt (ThinPrep) vial with appropriate patient information.

 b. Uncap PreservCyt vial. Remove pink cap from Evalyn brush.

 c. Depress the pink plunger on the Evalyn brush to expose the brush (white bristles).

 d. Vigorously plunge the brush, smashing the white brush against the bottom and interior wall of the vial 10 times to maximize sample release. Be careful not to splash.

 e. Discard Evalyn brush

 f. Tightly recap the PreservCyt vial.

 g. Submit the PreservCyt vial for testing.